Maximum intensity projections

The maximum intensity projection transform allows you to select a dimensional axis and computes the maximum intensity projection across all image planes along that axis. To do it, first import your dataset as described in the Importing a dataset from disk topic. Select your data from the list, then click the "Add >" button and choose "Maximum intensity projection" from the popup menu.

Choose a name for the maximum intensity projection, and VisBio will create another data object, a child of your original dataset, that represents the projected data. To alter which dimension is projected, select the projected data object and click the "Edit" button. A window similar to this one will appear:

Maximum intensity projection parameters

After choosing a dimensional axis to project from the pulldown menu, click the "Apply" button to lock in your changes. VisBio will recompute thumbnails as necessary for the new projection parameters.

For example, say you have imported a 4D dataset with 85 time points and 33 focal planes of color images (three range components: Red, Green and Blue). If you project the slice dimension, the resultant data object will have 85 time points, with 3 range components, with each image plane containing the maximum intensity of each pixel across all focal planes at that time point.

See also: